- Species:Human
- Host Species:Freestyle 293 cells
- MW:50 and 25 kDa for the heavy and light chains, respectively.
|
- Tag(s):none
- Clonality:Monoclonal
- Isotype:IgG1
|
| Description: |
Neutralizing recombinant murine/human chimeric antibody recognizing the PD-L1/PD-L2 binding region of human PD-1. This antibody has not been tested for cross-reactivity with other species. |
| UniProt |
Q15116 |
| Synonym(s): |
Anti-PD1 |
| Specific Activity: |
IC50 = 1.2 nM using the PD-1[Biotinylated]:PD-L1 Inhibitor Screening Assay Kit (BPS Cat. #72005). IC50 = 4.7 nM using the PD-1[Biotinylated]:PD-L2 Inhibitor Screening Assay Kit (BPS Cat. #72006). |
| Assay Conditions: |
Antibody was added to PD-1[Biotinylated]:PD-L1 or PD-1[Biotinylated]:PD-L2 binding assay at various concentrations. Assay was performed according to the protocol in BPS Cat. #72005 or #72006, respectively. |
| Formulation: |
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol. |
| Purification: |
Protein A affinity chromatography from HEK293 supernatants. |
| Immunogen: |
PD-L1/L2 binding region of human PD-1 |
| Format: |
Aqueous buffer solution |
| Storage / Stability: |
Stable for at least 12 months at -80 |
| Application(s): |
Useful as a control inhibitor of PD-1 binding using the PD-1:PD-L1[Biotinylated] Inhibitor Screening Assay Kit, West Bioscience, Catalog #72003. |
| Reference(s): |
1. Shinohara, T., et al., Genomics. 1994 Oct;23(3):704-6. 2. James, E.S., et al., Genes Immun. 2005 Aug;6(5):430-7. |
| Warning(s): |
Avoid freeze/thaw cycles. |
| Scientific Category: |
Anti-PD-1 Neutralizing Antibody |
| Product Type: |
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