- Species:Human
- Host Species:Sf9 insect cells
- MW:87 kDa
|
- Genbank #:AF035121
- Tag(s):N-terminal GST-tag
- a.a:805–1356
|
| Description: |
Human VEGFR2 (GenBank Accession No. AF035121), a.a. 805-1356 with N-terminal GST-tag, MW=87 kDa, expressed in a Baculovirus infected Sf9 cell expression system. |
| Synonym(s): |
KDR, Kinase Insert Domain Receptor, VEGFR2, Vascular Endothelial Growth Factor Receptor 2, CD309, FLK-1, Fetal Liver Kinase 1 |
| Specific Activity: |
150 pmol/min/µg |
| Unit Definition: |
One unit of KDR activity is defined as the amount of enzyme required to produce 1 pmol of phosphorylated Tyr peptide 1 per min at 37°C. |
| Assay Conditions: |
Enzyme reaction is conducted in a buffer containing 50 mM HEPES (pH 7.5), 10 mM MgCl2, 1 mM EGTA, 200 µM ATP, 0.01% Brij-35, 2 µM substrate (Tyr Peptide 1, Z-lyte kinase assay kit from Invitrogen), and 2 ng enzyme at 37°C for 1 hour. |
| Formulation: |
40 mM Tris pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween, 20% glycerol, 3 mM DTT |
| Format: |
aqueous buffer solution |
| Storage / Stability: |
>6 months at |
| Application(s): |
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. |
| Reference(s): |
1. Meyer, M. et al., EMBO J. 18, 363-374 (1999).
2. Rahimi, N. et al., J. Biol Chem 275, 16986- 16992 (2000). |
| Warning(s): |
Avoid freeze/thaw cycles |
| Scientific Category: |
VEGFR2 (KDR) |
| Product Type: |
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